Effect of Telang Flower Infusion on Lymphocyte Cells and Neutrophil Cells (Segment And Band) in Microplastic-Induced Rats

Authors

  • Ahdiah Imroatul Muflihah Medical Laboratory Technology, Faculty of Health Science, Universitas dr. Soebandi
  • Rian Anggia Destiawan Medical Laboratory Technology, Faculty of Health Science, University dr. Soebandi
  • Leny Yulia Widia Sari Medical Laboratory Technology, Faculty of Health Science, University dr. Soebandi

DOI:

https://doi.org/10.36858/jkds.v14i1.1057

Keywords:

Microplastics, lymphocyte cells, Telang flower, Segment neutrophil cells, Band neutrophil cells

Abstract

Background:   Microplastics causing tissue damage and increasing the number of lymphocyte cells and neutrophils (segment and band). Telang flower infusion contains flavonoids as antioxidants that can as immunomodulatory. The purpose of this study was to determine the effect of telang flower infusion on the number of lymphocyte cells, band and segment neutrophils. Methods:   Methode begins with the manufacture of telang flower infusion, the manufacture of microplastics (heating bottled drinking water at a temperature of 65oC), the analysis of the size of microplastics using electron microscopes, the analysis of microplastic types using the FTIR method, the grouping of negative control, positive control, and treatment with a dose of 4 ml/250g BW, exposure to microplastics, blood smear, giemsa staining for lymphocyte and neutrophil cell analysis, and statistical test using One-Way ANOVA (p<0.05) with Tuckey HSD (p<0.05). Results:  The results showed that the average number of negative control band neutrophil cells (3.50+4.50), positive control (3.17+3.81), and treatment (5.33+3.61) and the One-Way ANOVA analysis showed values (p=0.81>p=0.05), the average segment neutrophil cells of the negative control (23.67+6.80), the positive control (21.33+5.82), the treatment (24.00+8.19),  and the One-Way ANOVA analysis showed values (p=0.776>p=0.05), negative control lymphocyte cells (68.50+14.84), positive control (72.17+4.99), and treatment (64.00+12.10). The One-Way ANOVA analysis showed a value of (p=0.481>p=0.05). Conclusions:  It can be concluded that telang flower infusion has a tendency to act as an immunomodulator at the biological level characterized by a decrease in lymphocyte cells, an increase in band neutrophil cells and segment nuetrophic cells.

References

Ahmed, N., Tabassum, N., Rashid, P. T., Deea, B. J., Richi, F. T., Chandra, A., Agarwal, S., Mollick, S., Dipto, K. Z., Mim, S. A., & Alam, S. (2024). Clitoria ternatea L. (Butterfly Pea) Flower Against Endometrial Pain: Integrating Preliminary In Vivo and In Vitro Experimentations Supported by Network Pharmacology, Molecular Docking, and Molecular Dynamics Simulation Studies. In Life (Vol. 14, Number 11). https://doi.org/10.3390/life14111473

Aini, N., Khasanah, H., Husen, F., & Yuniati, N. I. (2023). Pewarnaan Sediaan Apusan Darah Tepi (SADT) Menggunakan Infusa Bunga Telang (Clitorea ternatea). Jurnal Bina Cipta Husada Vol. XIX(1), 67–76.

Amran, N. H., Zaid, S. S., Mokhtar, M. H., Manaf, L. A., & Othman, S. (2022). Exposure to Microplastics during Early Developmental Stage: Review of Current Evidence. In Toxics (Vol. 10, Number 10). https://doi.org/10.3390/toxics10100597

Hirt, N., & Body-Malapel, M. (2020). Immunotoxicity and intestinal effects of nano- and microplastics: a review of the literature. Particle and Fibre Toxicology, 17(1), 1–22. https://doi.org/10.1186/s12989-020-00387-7

Lee, Y., Sung, M., Sung, S.-E., Choi, J.-H., Kang, K.-K., Park, J. W., Kim, Y., & Lee, S. (2025). The histopathological and functional consequences of microplastic exposure. Discover Applied Sciences, 7(1), 72. https://doi.org/10.1007/s42452-025-06470-y

Liu, Z., Yu, P., Cai, M., Wu, D., Zhang, M., Chen, M., & Zhao, Y. (2019). Effects of microplastics on the innate immunity and intestinal microflora of juvenile Eriocheir sinensis. Science of The Total Environment, 685, 836–846. https://doi.org/https://doi.org/10.1016/j.scitotenv.2019.06.265

Malengier-Devlies, B., Metzemaekers, M., Wouters, C., Proost, P., & Matthys, P. (2021). Neutrophil Homeostasis and Emergency Granulopoiesis: The Example of Systemic Juvenile Idiopathic Arthritis. Frontiers in Immunology, Volume 12. https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.766620

Mehrani, Y., Morovati, S., Keivan, F., Tajik, T., Forouzanpour, D., Shojaei, S., Bridle, B. W., & Karimi, K. (2025). Dendritic Cells and Their Crucial Role in Modulating Innate Lymphoid Cells for Treating and Preventing Infectious Diseases. In Pathogens (Vol. 14, Number 8, p. 794). https://doi.org/10.3390/pathogens14080794

Muflihah, A. I. (2024). Efek Induksi Mikroplastik Terhadap Jumlah Neutrofil dan Limfosit Pada Mencit (Mus musculus L.). Journal of Nursing and Health, 9, 449–455. https://doi.org/https://doi.org/10.52488/jnh.v9i4.429

Multisona, R. R., Shirodkar, S., Arnold, M., & Gramza-Michalowska, A. (2023). Clitoria ternatea Flower and Its Bioactive Compounds: Potential Use as Microencapsulated Ingredient for Functional Foods. Applied Sciences (Switzerland), 13(4). https://doi.org/10.3390/app13042134

Park, K.-M., Kim, B., Woo, W., Kim, L. K., & Hyun, Y.-M. (2024). Polystyrene microplastics induce activation and cell death of neutrophils through strong adherence and engulfment. Journal of Hazardous Materials, 480, 136100. https://doi.org/https://doi.org/10.1016/j.jhazmat.2024.136100

Poosrisom, S., Wongfaed, N., Jumpa, T., Chiangjong, W., Xia, A., Reungsang, A., & Sittijunda, S. (2025). The influence of mixture proportions of polypropylene, polyvinyl chloride, and polyester microplastics on methane production: Integrative analysis of microbial diversity, enzymatic and metabolic profiles. Process Safety and Environmental Protection, 202, 107752. https://doi.org/https://doi.org/10.1016/j.psep.2025.107752

Prapanchan, V. N., Kumar, E., Subramani, T., Sathya, U., & Li, P. (2023). A Global Perspective on Microplastic Occurrence in Sediments and Water with a Special Focus on Sources, Analytical Techniques, Health Risks, and Remediation Technologies. Water (Switzerland), 15(11). https://doi.org/10.3390/w15111987

Putri, R. E., Sri Andayani, & Seto Sugianto Prabowo Rahardjo. (2025). Effect of Butterfly Pea (Clitoria ternatea) Flower Fraction on the To-tal Leukocyte Count of Common Carp (Cyprinus carpio). Journal of Aquaculture and Fish Health, 14(3), 412–426. https://doi.org/10.20473/jafh.v14i3.72195

Rosales, C. (2020). Neutrophils at the crossroads of innate and adaptive immunity. Journal of Leukocyte Biology, 108(1), 377–396. https://doi.org/https://doi.org/10.1002/JLB.4MIR0220-574RR

Sun, R., Xu, K., Yu, L., Pu, Y., Xiong, F., He, Y., Huang, Q., Tang, M., Chen, M., Yin, L., Zhang, J., & Pu, Y. (2021). Preliminary study on impacts of polystyrene microplastics on the hematological system and gene expression in bone marrow cells of rat. Ecotoxicology and Environmental Safety, 218, 112296. https://doi.org/10.1016/j.ecoenv.2021.112296

Supriyo, E., & Noviana, S. N. (2023). Kandungan Mikroplastik Pada Air Minum Dalam Kemasan (AMDK) yang Beredar di Semarang, Jawa Tengah. Metana, 19(2), 69–78. https://doi.org/10.14710/metana.v19i2.58548

Tamargo, A., Molinero, N., Reinosa, J. J., Alcolea-Rodriguez, V., Portela, R., Bañares, M. A., Fernández, J. F., & Moreno-Arribas, M. V. (2022). PET microplastics affect human gut microbiota communities during simulated gastrointestinal digestion, first evidence of plausible polymer biodegradation during human digestion. Scientific Reports, 12(1), 528. https://doi.org/10.1038/s41598-021-04489-w

Wang, L., Liang, Y., Zhao, C., Ma, P., Zeng, S., Ju, D., Zhao, M., Yu, M., & Shi, Y. (2025). Regulatory T cells in homeostasis and disease: molecular mechanisms and therapeutic potential. Signal Transduction and Targeted Therapy, 10(1), 345. https://doi.org/10.1038/s41392-025-02326-4

Wang, R., Lan, C., Benlagha, K., Camara, N. O. S., Miller, H., Kubo, M., Heegaard, S., Lee, P., Yang, L., Forsman, H., Li, X., Zhai, Z., & Liu, C. (2024). The interaction of innate immune and adaptive immune system. MedComm, 5(10), e714. https://doi.org/https://doi.org/10.1002/mco2.714

Yang, W., Li, Y., & Boraschi, D. (2023). Association between Microorganisms and Microplastics: How Does It Change the Host–Pathogen Interaction and Subsequent Immune Response? International Journal of Molecular Sciences, 24(4). https://doi.org/10.3390/ijms24044065

Zhao, L., Shi, W., Hu, F., Song, X., Cheng, Z., & Zhou, J. (2021). Prolonged oral ingestion of microplastics induced inflammation in the liver tissues of C57BL/6J rat through polarization of macrophages and increased infiltration of natural killer cells. Ecotoxicology and Environmental Safety, 227, 112882. https://doi.org/https://doi.org/10.1016/j.ecoenv.2021.112882

Zheng, Y., Li, J., Cao, W., Jiang, F., Zhao, C., Ding, H., Wang, M., Gao, F., & Sun, C. (2020). Vertical distribution of microplastics in bay sediment reflecting effects of sedimentation dynamics and anthropogenic activities. Marine Pollution Bulletin, 152, 110885. https://doi.org/https://doi.org/10.1016/j.marpolbul.2020.110885

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Published

2026-04-30

How to Cite

Muflihah, A. I., Destiawan, R. A., & Sari, L. Y. W. (2026). Effect of Telang Flower Infusion on Lymphocyte Cells and Neutrophil Cells (Segment And Band) in Microplastic-Induced Rats. Jurnal Kesehatan Dr. Soebandi, 14(1), 89–98. https://doi.org/10.36858/jkds.v14i1.1057

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Vol. 14 No. 1 (2026): Jurnal Kesehatan dr. Soebandi

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