In Vitro Inhibition Activity of Elastase and and Tyrosinase Enzymes of Parijoto Fruit (Medinilla speciosa) Extract and Fractions
DOI:
https://doi.org/10.36858/jkds.v14i1.835Keywords:
Medinilla speciosa, Extract, Fraction, AntiagingAbstract
Background : Ultraviolet rays and pollution cause premature aging of the skin, darkening, dryness, and decreased elasticity. Parijoto fruit (Medinilla speciosa) offers potential for development as an anti-aging agent. Methods : The current research was conducted to investigate the influence of Extended Pharmaceutical Marketing Mix (product, price, place, promotion, pharmacist, process and physical evidence) on customer satisfaction at pharmacies in Jember. . Four fractions were obtained through liquid-liquid partition using n-hexane, ethyl acetate, butanol, and water solvents. Elastase and tyrosinase enzyme inhibition tests were carried out in vitro using an ELISA (multi-mode reader). The reference compound in the elastase enzyme inhibition test was gallic acid, and kojic acid was used to inhibit the tyrosinase enzyme. Results: The elastase enzyme inhibitory activity of the extract and four fractions and gallic acid with a concentration of 133.33 μg/mL were 13.28; 21.56; 14.88; 15.12; 11.24; and 21.83%. The tyrosinase inhibitory activity of parijoto fruit extract and four fractions and kojic acid with a concentration of 125 μg/mL were 17.01; 19.2; 23.96; 14.76; 18.20; 34.71%, respectively. Parijoto fruit extract and fractions had elastase and tyrosinase inhibitory activity. Conclusions: The n-hexane fraction was the active fraction in inhibiting the elastase enzyme by 21.56%, while the active fraction in inhibiting the tyrosinase enzyme was the ethyl acetate fraction which provided an activity of 23.96%.
References
Ameliya, Setyowati, E., & Besan, E. J. (2025). Analisis Kemometrika Kandungan Fitokimia Dengan Aktivitas Antiacne Ekstrak Buah Parijoto (Medinilla speciosa Blume). Nusantara Hasana Journal, 5(2), 282–294.
Brahmanti, H., & Dyah Puspitasari, G. (2023). Peran Elastin Dalam Proses Photoaging Kulit. Journal of Dermatology, Venereology, and Aesthetic, 41–51.
Cahyaningsih, E., Yuda, P. E. S. K., & Santoso, P. (2019). Skrining Fitokimia dan Uji Aktivitas Antioksidan Ekstrak Etanol Bunga Telang (Clitoria ternatea L.) Dengan Metode Spektrofotometri UV-VIS. Jurnal Ilmiah Medicamento, 5(1), 51–57. https://doi.org/10.36733/medicamento.v5i1.851
Chen, X., Shang, S., Yan, F., Jiang, H., Zhao, G., Tian, S., Chen, R., Chen, D., & Dang, Y. (2023). Antioxidant Activities of Essential Oils and Their Major Components in Scavenging Free Radicals, Inhibiting Lipid Oxidation and Reducing Cellular Oxidative Stress. Molecules, 28(11), 1–10. https://doi.org/10.3390/molecules28114559
Dini, P. P., Nurohmi, S., Aisya, R. W., Sukoharjanti, B. T., Inas, Z., & Hartanto, F. S. (2024). Education Food Sources of Antioxidants and Optimizing Skincare to Prevent Premature. 11, 2722–2729.
Ernawati, E. E. (2024). Standardisasi Ekstrak Buah Ceremai (Phyllanthus Acidus L. Skeels) dan Aktivitas Inhibisi Enzim Elastase. Jurnal Kartika Kimia, 6(2), 145–155. https://doi.org/10.26874/jkk.v6i2.225
Eun Lee, K., Bharadwaj, S., Yadava, U., & Gu Kang, S. (2019). Evaluation of caffeine as inhibitor against collagenase, elastase and tyrosinase using in silico and in vitro approach. Journal of Enzyme Inhibition and Medicinal Chemistry, 34(1), 927–936. https://doi.org/10.1080/14756366.2019.1596904
Furi, M., Ainun Alfatma, Rahma Dona, Armon Fernando, Fina Aryani, Rahayu Utami, Septi Muharni, Husnawati, Wira Noviana Suhery, & Melzi Octaviani. (2022). Uji Inhibitor Enzim Tirosinase Ekstrak dan Fraksi Daun Kedabu (Sonneratia ovata Backer) Secara In-Vitro. Jurnal Ilmiah Manuntung, 8(2), 201–214. https://doi.org/10.51352/jim.v8i2.529
Gazali, M., P. Zamani, N., & Batubara, I. (2014). Potensi limbah kulit buah Nyirih Xylocarpus granatum sebagai inhibitor tirosinase. Depik, 3(3), 187–194. https://doi.org/10.13170/depik.3.3.2153
Hadi, F., Agustin, D., & Cahyani, F. (2015). Uji Toksisitas Tanin dari Kulit Manggis (Garcinia mangostana L.) terhadap SelFibroblas BHK - 21. Conservative Dentistry Journal, 5(1), 6–11.
Hanani, E. (2015). Analisis Fitokimia. Buku Kedokteran EGC.
Handayani, V. (2014). Isolasi dan Identifikasi Komponen Kimia Ekstrak n-Heksan Daun Pala (Myristica fragrans). Jurnal Ilmiah As-Syifaa, 6(2), 135–144. https://doi.org/10.33096/jifa.v6i2.43
Hidayah, W. W., Kusrini, D., & Fachriyah, E. (2016). Isolasi, Identifikasi Senyawa Steroid dari Daun Getih-Getihan (Rivina humilis L.) dan Uji Aktivitas sebagai Antibakteri. Jurnal Kimia Sains dan Aplikasi, 19(1), 32. https://doi.org/10.14710/jksa.19.1.32-37
Jakimiuk, K., Gesek, J., Atanasov, A. G., & Tomczyk, M. (2021). Flavonoids as inhibitors of human neutrophil elastase. Journal of Enzyme Inhibition and Medicinal Chemistry, 36(1), 1016–1028. https://doi.org/10.1080/14756366.2021.1927006
Jiratchayamaethasakul, C., Ding, Y., Hwang, O., Im, S. T., Jang, Y., Myung, S. W., Lee, J. M., Kim, H. S., Ko, S. C., & Lee, S. H. (2020). In vitro screening of elastase, collagenase, hyaluronidase, and tyrosinase inhibitory and antioxidant activities of 22 halophyte plant extracts for novel cosmeceuticals. Fisheries and Aquatic Sciences, 23(1), 1–9. https://doi.org/10.1186/s41240-020-00149-8
Kim, J. H., Leem, H. H., & Lee, G. Y. (2020). The guanidine pseudoalkaloids 10‐methoxy‐ leonurine and leonurine act as competitive inhibitors of tyrosinase. Biomolecules, 10(2). https://doi.org/10.3390/biom10020174
Kim, J. Y., Lee, J. H., Song, Y. H., Jeong, W. M., Tan, X., Uddin, Z., & Park, K. H. (2015). Human neutrophil elastase inhibitory alkaloids from chelidonium majus L. Journal of Applied Biological Chemistry, 58(3), 281–285. https://doi.org/10.3839/jabc.2015.044
Marinaccio, L., Stefanucci, A., Scioli, G., Valle, A. Della, Zengin, G., Cichelli, A., & Mollica, A. (2022). Peptide Human Neutrophil Elastase Inhibitors from Natural Sources : An Overview. International Journal of Molecular Sciences, 23.
Mona, N., Widyastuti, D. A., Nurwahyunani, A., & Nurdyansyah, F. (2022). Fraksinasi Ekstrak Metanol Buah Parijoto (Medinilla speciosa Blume) dengan Pelarut Metanol, Etil Asetat, dan N-Heksana. UNIVERSITAS PGRI MADIUN, Vol 9, No.
Natanael, G. I., Simorangkir, G. F., Parariski, N., Putri, M., Tambunan, B., Amansyah, A., & Nasution, A. N. (2021). Potensi Antioksidan Dan Anti-Elastase Ekstrak Daun Kelor (Moringa oleifera) Terhadap Antiaging. Jurnal Keperawatan Priority, 4(1), 69–76.
Normaidah, N., Rahmah, M., Izma, H., Fadlilaturrahmah, F., & Sari, A. K. (2024). The Antityrosinase Activity of Mitragyna speciosa Korth Leaf Ethanolic Extract. Jurnal Ilmiah Kesehatan (JIKA), 6(1), 129–138. https://doi.org/10.36590/jika.v6i1.761
Oentari, O. D. (2023). Uji Aktivitas Antibakteri Fraksi N-Heksan, Etil Asetat, Metanol-Air Dari Ekstrak Daun Jambu Mede ( Anacardium occidentale Linn ) TERHADAP Pseudomonas aeruginosa ATCC 27853. Jurnal Kesehatan Tujuh Belas (Jurkes 17), 4(2), 264–268.
Pittayapruek, P., Meephansan, J., Prapapan, O., Komine, M., & Ohtsuki, M. (2016). Role of matrix metalloproteinases in Photoaging and photocarcinogenesis. International Journal of Molecular Sciences, 17(6). https://doi.org/10.3390/ijms17060868
Putri, F. E., Diharmi, A., & Karnila, R. (2023). Identifikasi Senyawa Metabolit Sekunder Pada Rumput Laut Coklat (Sargassum plagyophyllum) Dengan Metode Fraksinasi. Jurnal Teknologi dan Industri Pertanian Indonesia, 15(1), 40–46. https://doi.org/10.17969/jtipi.v15i1.23318
Ramadhani, S., Elya, B., & Forestrania, R. C. (2023). Aktivitas Anti-elastase dan Antioksidan dari Ekstrak Etanol Kayu Bangkal (Nauclea subdita) Korth. Steud. dengan Variasi Metode Ekstraksi. Jurnal Mandala Pharmacon Indonesia, 9(2), 228–243. https://doi.org/10.35311/jmpi.v9i2.347
Rauf, A. (2016). 50 78,89. potensi penghambatan tirosinase ekstrak etanol daun tomat (Lycopersicon esculentum Mill, var. pyriforme Alef).
Riantica, E., Nurhayati, A. P. D., & Sa’adah, N. N. (2023). An activity of flavonoid derived compounds from Medinilla speciosa as anti-hyperpigmentation against tyrosinase proteins with in silico methods. International Journal of Science and Research Archive, 10(2), 1113–1123. https://doi.org/10.30574/ijsra.2023.10.2.1090
Saepudin, S., Hesty Nuur Hanifah, Kusdi Hartono, Lia Mutiara, & Didit Andita. (2024). Profil Kromatografi Lapis Tipis dan Aktivitas Tabir Surya Ekstrak Etanol 70% Daun Kesumba (Polygonum minus Huds). Jurnal Ilmiah Farmasi Farmasyifa, 7(2), 192–203. https://doi.org/10.29313/jiff.v7i2.3667
Sagala, Z., & Ripaldo, F. (2020). Uji Aktivitas Inhibitor Enzim Tirosinase Dan Uji Antioksidan Ekstrak Etanol Buah Harendong (Melastoma malabathricum L.) Secara In Vitro. Indonesia Natural Research Pharmaceutical Journal, 5(1), 1–16.
Sholikhati, A., Dwi Kurnia, S., & Mundriyastutik, Y. (2024). Kajian Aktivitas Farmakologis Buah Parijoto (Medinilla Speciosa): Sistematik Review. Jurnal Medika Indonesia, 5(1), 27–32.
Sinulingga, C. N., Warsidah, Sofiana, M. S. J., & Safitri, I. (2022). Uji Aktivitas Penghambatan Enzim Alfa-Tirosinase Sebagai Bahan Kosmetik. Oseanologia, 1(2), 50–54.
Tinasari, N. D., Vifta, R. L., Farmasi, P. S., Waluyo, U. N., Farmasi, P. S., Islam, U., & Agung, S. (2024). Potensi Ekstrak Buah Parijoto ( Medinilla speciosa Blume ) Sebagai Bahan Aktif Sediaan Antioksidan. 1.
Wendersteyt, N. V., Wewengkang, D. S., & Abdullah, S. S. (2021). Antimicrobial Activity Test Of Exstracts And Fractions Of Ascidian herdmania momus From Bangka Island Waters Likupang Against The Growth Of Staphylococcus aureus, Salmonella typhimurium, And Candida albicans. Pharmacon, 10(1), 706. https://doi.org/10.35799/pha.10.2021.32758
Winanta, A., Sari, G. A., Febriansah, R., & Nugraha, A. T. (2024). Antioxidant and Anti-aging Activity of Parijoto Fruit (Medinilla Speciosa, Reinw.ex Bl.) Ethanolic Extract In Vitro (Nomor Ichse). Atlantis Press International BV. https://doi.org/10.2991/978-94-6463-431-0_5
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