Phenolic Assay and Antioxidant Activity of Ethyl Acetate and Water Fractions of Ethanol Extract of Butterfly Pea Flower by DPPH  and FRAP Methods

Dewi Fitriani; Rani Nur Ayu Utami; Rina Nur Ayu Ta’ati

doi:10.36858/jkds.v13i2.845

Authors

Dewi Fitriani STIFAR YAYASAN PHARMASI SEMARANG
Rani Nur Ayu Utami STIFAR Yayasan Pharmasi Semarang
Rina Nur Ayu Ta’ati STIFAR Yayasan Pharmasi Semarang

DOI:

https://doi.org/10.36858/jkds.v13i2.845

Keywords:

Antioxidant, Butterfly pea flower, DPPH, FRAP

Abstract

Antioxidants play an important role in human life. Many studies have been conducted to discover the potential of antioxidants sourced from nature. Several methods can be used to test antioxidants. These methods include FRAP, ABTS, and DPPH. All three methods are widely used because they are stable and relatively easy to use. Several studies on TAC (Total Antioxidant Capacity) have reported different results regarding antioxidant testing using different methods on the same material. This study aims to test the ethyl acetate and water fractions of butterfly pea flower extract using the DPPH and FRAP methods and to compare the results with the findings of previous researchers who tested using the ABTS method. The research method began with the extraction of butterfly pea flowers using 70% ethanol solvent, followed by a total phenolic test using the Folin Ciocalteu method. Next, fractionation was carried out, and the ethyl acetate and water fractions were then tested for antioxidant activity using the DPPH method, while FRAP was performed for the ethyl acetate fraction. Quercetin was used as a reference standard. The results of the antioxidant activity test of butterfly pea flowers using the DPPH method showed an IC50 value of 27.63 ppm ±4.11 in the ethyl acetate fraction and 54.70 ppm ±2.22 in the water fraction, while the IC50 in the FRAP method was 97.47 ppm ±3.2 in the ethyl acetate fraction. DPPH and ABTS did not show significant differences in the antioxidant activity test results, but showed different results in FRAP.

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