The Patch Formulation of Rambusa (Passiflora foetida) Leave Extract as Anti-Inflammatory Agent: An In Vitro and In Vivo Study

Lindawati Setyaningrum; Aliyah Purwanti; Widaddhiya Zahra Anggraini; Adelia Royan Suryani; Tolak Haris; Lubis Nuril Ubaidilla

doi:10.36858/jkds.v12i1.712

Authors

Lindawati Setyaningrum Pharmacy Program, Faculty of Health Sciences, Universitas dr. Soebandi
Aliyah Purwanti Pharmacy Program, Faculty of Health Sciences, Universitas dr. Soebandi
Widaddhiya Zahra Anggraini Pharmacy Program, Faculty of Health Sciences, Universitas dr. Soebandi
Adelia Royan Suryani Pharmacy Program, Faculty of Health Sciences, Universitas dr. Soebandi
Tolak Haris Pharmacy Program, Faculty of Health Sciences, Universitas dr. Soebandi
Lubis Nuril Ubaidilla Pharmacy Program, Faculty of Health Sciences, Universitas dr. Soebandi

DOI:

https://doi.org/10.36858/jkds.v12i1.712

Keywords:

Anti-inflammatory, Rambusa Leave, Invitro, Invivo, Patch

Abstract

Rambusa leaves are known to have health benefits, including anti-inflammatory, antitumor, anticancer, antihepatotoxicity and antimicrobial. Rambusa leaves contain active compounds including alkaloids, tannins, saponins, and steroids which have anti-inflammatory effects. So it was developed as a transdermal route preparation in the form of a patch to overcome the problem of side effects from using oral medications on the gastrointestinal tract. Rambusa leaves are formulated into additional ingredients in the form of HPMC, methyl paraben, propylene glycol, 96% ethanol, distilled water and made into ready-to-use patches. Furthermore, an evaluation of the physical quality of the formula was carried out and its anti-inflammatory effectiveness was determined through in vitro and in vivo tests. The results show that each formula produces physical properties that meet the requirements. Apart from that, the results of the in vitro test using sodium diclofenac as a standard show that formulas 100, 200 and 300 are able to penetrate the membrane from the results of the cumulative amount of active ingredients that have been tested, whereas in the in vivo test using wistar rat test, the results of % edema were analyzed using ANOVA and the results of % inhibition were carried out in a post hoc test with LSD. The highest effectiveness was obtained in formula 300, this shows that the formula is capable of inhibiting inflammation. From these results it can be concluded that rambusa leave extract can be developed into a transdermal patch formula for anti-inflammation.

References

Arifin, A., Muhammad Iqbal. (2019). FORMULASI DAN UJI KARAKTERISTIK FISIK SEDIAAN PATCHEKSTRAK ETANOLDAUNKUMIS KUCING (Orthosiphon stamineus). Jurnal Ilmiah Manuntung, 5(2), 187-191.

Arum, M., Wahyuningsih, S., & Amin, R. (2022). Uji Efektivitas Patch Transdermal Ekstrak Etanol Kulit Batang Kayu Jawa (Lannea Coromandelica (Houtt.) Merr) Terhadap Luka Sayat Pada Tikus Putih Jantan (Rattus Norvegicus). Jurnal Multidisiplin Madani, 2(2), 1001-1018.

Chandra, D. (2019). Pengujian Penetrasi In-Vitro Sediaan Gel, Krim, Gel-Krim Ekstrak Biji Kopi (Coffea arabica L.) sebagai Antiselulit. JIFI (Jurnal Ilmiah Farmasi Imelda), 3(1), 14-21.

Ermawati, D. E., dan Heni Utami Prilantari. (2019). Pengaruh Kombinasi Polimer Hidroksipropilmetilselulosa dan Natrium Karboksimetilselulosa terhadap Sifat Fisik Sediaan Matrix-based Patch Ibuprofen. JPSCR: Journal of Pharmaceutical Science and Clinical Research, 4(2), 109-119.

Fatmawaty, A., Nisa, M., Irmayani, I., & Sunarti, S. (2017). Formulasi patch ekstrak etanol daun murbei (Morus alba L.) dengan variasi konsentrasi polimer polivinil pirolidon dan etil selulosa. Journal of Pharmaceutical and Medicinal Sciences, 2(1).

Febrianti, D. R., & Musiam, S. (2019). Potensi Kombinasi Kapur Sirih dan Daun Kumpai Mahung (Eupatorium inulifolium HB &K.) sebagai Alternatif Salep Anti Inflamasi Alami. Jurnal Ilmiah Ibnu Sina, 4(2), 323-330.

Hartesi, B., Desi Sagita, Lili Andriani, Tendri Angke, Serly Natalia. (2021). PATCH TRANSDERMAL DARI FRAKSI N-HEKSAN EKSTRAK RUKU-RUKU (OCIMUM TENUIFLORUM L.) SEBAGAI ANTIINFLAMASI. Jurnal Endurance : Kajian Ilmiah Problema Kesehatan, 6(2), 250-262.

Kaluku, R. I., Tungadi, R., & Thomas, N. A. (2022). Effect of HEC (Hydroxyethyl Cellulose) Polymer on Nanoemulsion-Based Curcumin Transdermal Patch Release. Indonesian Journal of Pharmaceutical Education, 2(3), 197-207.

Kusuma, M. P., & Suparno, N. R. (2021). POTENSI PATCH MUKOADHESIF KOMBINASI DAUN SIRIH HIJAU, GAMBIR, DAN BIJI PINANG PADA PENYAKIT PERIODONTAL. JIKG (Jurnal Ilmu Kedokteran Gigi), 4(1), 29-34.

Li S, L. X., Chen X, Bi L. (2020). Research Progress on Anti-Inflammatory Effects and Mechanisms of Alkaloids from Chinese Medical Herbs. Evidence-Based Complementary and Alternative Medicine.

Manasadeepa, R., Paul, P., & Mukherjee. (2013). Pressure-sensitive mucoadhesive polymer-based dental patches to treat periodontal diseases: an in vitro study. Drug Delivery, 20(6), 258-267.

Mauludiyah, M. (2018). Kajian Potensi Liposom Ekstrak Daun Dan Tangkai Ciplukan (Physalis Minima) Dalam Sediaan Patch Sebagai Antiinflamasi Pada Model Hewan Inflamasi Akut Dan Kronik Universitas Brawijaya].

Mulyani, E. (2019). Studi In Vitro: Efek Anti Kolesterol Ekstrak Daun Rambusa (Passiflora foetida L): In Vitro Study: Anti-Cholesterol Effect of Rambusa Leaf Extract (Passiflora foetida L). Jurnal Surya Medika (JSM), 4(2), 60-65.

Mulyani, E., Suryadini, H., & Putri, R. R. (2022). Formulasi Sediaan Krim Antiinflamasi Ekstrak Etanol Daun Rambusa (Passiflora foetida L): Cream Formulation Antiinflamatory Ethanol Extracts of Rambusa (Passiflora foetida L). Jurnal Surya Medika (JSM), 7(2), 219-222.

Novia, N., & Noval, N. (2021). Pengaruh Kombinasi Polimer Polivinil Pirolidon dan Etil Selulosa terhadap Karakteristik dan Uji Penetrasi Formulasi Transdermal Patch Ekstrak Bawang Dayak (Eleutherine palmifolia (L): The Effect of Polyvinyl Pyrolidon and Ethyl Cellulose Polymer Combination on Characteristics and Penetration Test of Formulation Transdermal of Dayak Onion Extract Patch (Eleutherine palmifolia (L.). Jurnal Surya Medika (JSM), 7(1), 173-184.

Novian, D. R. (2022). Studi In Silico Potensi Antimalaria Passiflora Foetida. WELLNESS AND HEALTHY MAGAZINE, 4(1), 47-52.

Novita, P. S. (2021). FORMULASI SEDIAAN PATCH DENGAN VARIASI PEG 400 SEBAGAI PLASTISIZER MENGGUNAKAN EKSTRAK DAUN SELEDRI (Apium graveolens Linn.) Sekolah Tinggi Ilmu Kesehatan Nasional].

Nurahmanto, D., Nurul Shalikha, Lidya Ameliana. (2017). Optimasi Hidroksipropil Metilselulosa K-4M dan Carbopol® 940 pada Sediaan Patch Dispersi Padat Piroksikam. KARTIKA: JURNAL ILMIAH FARMASI, 5(2), 80-86.

Patel DP, S. C., Mistry GN, Patel SL, Patel TJ, Mistry PC, Rana AK, Patel PK, Mishra RS. (2009). Development and evaluation of ethyl cellulose-based transdermal films of furosemide for improved in vitro skin permeation. AAPS PharmSciTech, 10(2), 437.

Rahman, S., Amirah, S., Fitriana, F., & Putra, B. (2023). AKTIVITAS ANTIINFLAMASII EKSTRAK ETANOL BATANG WOLE WOE PADA TIKUS DENGAN METODE RAT HIND PAW EDEMA. As-Syifaa Jurnal Farmasi, 15(1), 30-36.

Sentat , T., Fitri Handayani. (2018). STUDY ON ANTI-INFLAMMATORY ACTIVITY OF ETHANOL EXTRACT OF WILD TAMARIND SEED (Leucaena leucocephala L.) IN CARRAGEENAN INDUCED MICE PAW EDEMA. JURNAL ILMU KESEHATAN, 6(1).

Sudhanshu Mishra, P. V., Saurabh Kumar Gupta, Sudhi Pandey, Smriti Ojha. (2022). Nanocarrier and Herbal Based Transdermal Patch: An Advantage Over Other Drug Delivery Systems. Annals of Ayurvedic Medicine, 11(2), 145-156.

Suryana, K. (2017). Corticosteroid Sistemik pada Penyakit Inflamasi. Improving Clinical Kkill and Knowledge and Comprehensive Management of Internal Medicine in Social Insurance Era, Denpasar.

Suryani, W. O. S. M., Aisyah Shaliha Anto. (2017). Optimasi Formula Matriks Patch Transdermal Nanopartikel Teofilin dengan Menggunakan Metode Simplex Lattice Design (SLD). Majalah Farmasi, Sains, dan Kesehatan, 3(1), 26-32.

Tan, B., Wu, X., Yu, J., & Chen, Z., . (2022). The Role of Saponins in the Treatment of Neuropathic Pain. Molecules 27(12), 3956.

Tong, Z., He, W., Fan, X., & Guo, A. (2022). Biological Function of Plant Tannin and Its Application in Animal Health [Review]. Frontiers in Veterinary Science, 8. https://doi.org/10.3389/fvets.2021.803657

Wardani, V. K., & Saryanti, D. (2021). Formulasi transdermal patch ekstrak etanol biji pepaya (Carica papaya L.) dengan basis Hydroxypropil Metilcellulose (HPMC). Smart Medical Journal, 4(1), 38-44.